Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human, Mouse, Fish - Danio rerio (Zebrafish)

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SREBP2 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 300 and 350 of human Sterol Regulatory Element Binding Transcription Factor 2 using the numbering given in entry NP_004590.2 (GeneID 6721).

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 24057001). Use in Mouse reported in scientific literature (PMID:28546220).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SREBP2 Antibody

Western Blot: SREBP2 Antibody [NBP1-71880]

Western Blot: SREBP2 Antibody [NBP1-71880]

Western Blot: SREBP2 Antibody [NBP1-71880] - Activation of Srebf pathways in slc16a6a mutant livers. After 45 days of feeding wild-type (WT) and mutant (MUT) animals ketogenic diets (K4 or K12), livers were harvested and analyzed. Immunoblot analysis of Srebf1, Srebf2, Acac, phospho-Ser80 Acac, Hmgcr and Btub (beta-tubulin) (five livers pooled per lane). For Srebf1, an additional nuclear isolation is shown (bottom) because maturation of this transcription factor is not as striking in whole lysates as it is for Srebf2. Image collected and cropped by CiteAb from the following publication (https://dmm.biologists.org/cgi/doi/10.1242/dmm.013425), licensed under a CC-BY license.
Immunoprecipitation: SREBP2 Antibody [NBP1-71880]

Immunoprecipitation: SREBP2 Antibody [NBP1-71880]

Immunoprecipitation: SREBP2 Antibody [NBP1-71880] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SREBP2 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SREBP2 was also immunoprecipitated by rabbit anti-SREBP2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for SREBP2 Antibody

Application
Recommended Usage

Immunoprecipitation

2- 10 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SREBP2

This gene encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain.

Long Name

Sterol Regulatory Element Binding Transcription Factor 2

Alternate Names

bHLhd2, SREBF2

Entrez Gene IDs

6721 (Human)

Gene Symbol

SREBF2

UniProt

Q12772 (Human)

Additional SREBP2 Products

Product Documents for SREBP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SREBP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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