SREBP2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71880
![Western Blot: SREBP2 Antibody [NBP1-71880]](https://resources.bio-techne.com/images/products/SREBP2-Antibody-Western-Blot-NBP1-71880-img0002.jpg "Western Blot: SREBP2 Antibody [NBP1-71880]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Zebrafish
Cited:
Human, Mouse, Fish - Danio rerio (Zebrafish)
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 300 and 350 of human Sterol Regulatory Element Binding Transcription Factor 2 using the numbering given in entry NP_004590.2 (GeneID 6721).
Reactivity Notes
Zebrafish reactivity reported in scientific literature (PMID: 24057001). Use in Mouse reported in scientific literature (PMID:28546220).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SREBP2 Antibody
Western Blot: SREBP2 Antibody [NBP1-71880]
Western Blot: SREBP2 Antibody [NBP1-71880] - Activation of Srebf pathways in slc16a6a mutant livers. After 45 days of feeding wild-type (WT) and mutant (MUT) animals ketogenic diets (K4 or K12), livers were harvested and analyzed. Immunoblot analysis of Srebf1, Srebf2, Acac, phospho-Ser80 Acac, Hmgcr and Btub (beta-tubulin) (five livers pooled per lane). For Srebf1, an additional nuclear isolation is shown (bottom) because maturation of this transcription factor is not as striking in whole lysates as it is for Srebf2. Image collected and cropped by CiteAb from the following publication (https://dmm.biologists.org/cgi/doi/10.1242/dmm.013425), licensed under a CC-BY license.![Immunoprecipitation: SREBP2 Antibody [NBP1-71880]](https://resources.bio-techne.com/images/products/SREBP2-Antibody-Immunoprecipitation-NBP1-71880-img0001.jpg "Immunoprecipitation: SREBP2 Antibody [NBP1-71880]")
Immunoprecipitation: SREBP2 Antibody [NBP1-71880]
Immunoprecipitation: SREBP2 Antibody [NBP1-71880] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SREBP2 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SREBP2 was also immunoprecipitated by rabbit anti-SREBP2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for SREBP2 Antibody
Application
Recommended Usage
Immunoprecipitation
2- 10 ug/mg lysate
Western Blot
1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SREBP2
Long Name
Sterol Regulatory Element Binding Transcription Factor 2
Alternate Names
bHLhd2, SREBF2
Entrez Gene IDs
6721 (Human)
Gene Symbol
SREBF2
UniProt
Additional SREBP2 Products
Product Documents for SREBP2 Antibody
Product Specific Notices for SREBP2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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