SRM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80726

Novus Biologicals, part of Bio-Techne
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NBP1-80726
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SRM Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FKESYYQLMKTALKEDGVLCCQGECQWLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEPVQPLTQQQVAQMQLKYYNSDVHRAAFVLPEF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRM Antibody

Western Blot: SRM Antibody [NBP1-80726]

Western Blot: SRM Antibody [NBP1-80726]

Western Blot: SRM Antibody [NBP1-80726] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SRM Antibody [NBP1-80726]

Immunocytochemistry/ Immunofluorescence: SRM Antibody [NBP1-80726]

Immunocytochemistry/Immunofluorescence: SRM Antibody [NBP1-80726] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SRM Antibody [NBP1-80726]

Immunohistochemistry-Paraffin: SRM Antibody [NBP1-80726]

Immunohistochemistry-Paraffin: SRM Antibody [NBP1-80726] - Staining of human stomach shows cytoplasmic positivity in glandular cells.

Applications for SRM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SRM

The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells. The human complex consists of seven subunits which include the actin related proteins Arp2 and Arp3, and five others referred to as p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc (p omplex) (1). These proteins localize in the cytoplasm of fibroblasts that lack lamellipodia, but are enriched in lamellipodia on stimulation with serum or platelet-derived growth factor. A dynamic role for this complex in the organization of the actin cytoskeleton has been suggested (2). p16-Arc has been found to be a globular alpha-helical subunit (3).

Alternate Names

EC 2.5.1, EC 2.5.1.16, Putrescine aminopropyltransferase, SPDSY, spermidine synthase, spermidine synthase-1, SPS1PAPT, SRML1

Entrez Gene IDs

6723 (Human)

Gene Symbol

SRM

UniProt

P19623 (Human)

Additional SRM Products

Product Documents for SRM Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SRM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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