SRP72 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97754
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SRP72 Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human SRP72 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SRP72 Antibody
Western Blot: SRP72 Antibody [NBP2-97754]
Western Blot: SRP72 Antibody [NBP2-97754] - Anti-SRP72 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 75 kDa. Observed band size: 75 kDaImmunocytochemistry/ Immunofluorescence: SRP72 Antibody [NBP2-97754]
Immunocytochemistry/Immunofluorescence: SRP72 Antibody [NBP2-97754] - Immunofluorescence staining of SRP72 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human SRP72 polyclonal antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Immunohistochemistry-Paraffin: SRP72 Antibody [NBP2-97754]
Immunohistochemistry-Paraffin: SRP72 Antibody [NBP2-97754] - Immunochemical staining of human SRP72 in human pancreas with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.Applications for SRP72 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SRP72
Alternate Names
signal recognition particle 72kDa
Gene Symbol
SRP72
Additional SRP72 Products
Product Specific Notices for SRP72 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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