SRPK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87050

Novus Biologicals, part of Bio-Techne
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NBP1-87050
NBP1-87050-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SRPK1 Antibodies »

Product Summary for SRPK1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEIEEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRPK1 Antibody

Western Blot: SRPK1 Antibody [NBP1-87050]

Western Blot: SRPK1 Antibody [NBP1-87050]

Western Blot: SRPK1 Antibody [NBP1-87050] - Analysis in control (vector only transfected HEK293T lysate) and SRPK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-87050]

Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-87050]

Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-87050] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: SRPK1 Antibody [NBP1-87050]

Western Blot: SRPK1 Antibody [NBP1-87050]

Western Blot: SRPK1 Antibody [NBP1-87050] - Analysis using Anti-SRPK1 antibody NBP1-87050 (A) shows similar pattern to independent antibody NBP2-58538 (B).

Applications for SRPK1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SRPK1

SRPK1 encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. A second alternatively spliced transcript variant for this gene has been described, but its full length nature has not been determined.

Long Name

SRSF protein kinase 1

Alternate Names

EC 2.7.11.1, Serine/arginine-rich protein-specific kinase 1, serine/arginine-rich splicing factor kinase 1, serine/threonine-protein kinase SRPK1, SFRS protein kinase 1SFRSK1, SR protein kinase 1, SR-protein-specific kinase 1, SRSF protein kinase 1

Gene Symbol

SRPK1

Additional SRPK1 Products

Product Documents for SRPK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SRPK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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