Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3C8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SRY Antibodies »

Product Summary for SRY Antibody (OTI3C8) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human SRY(NP_003131) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SRY Antibody (OTI3C8) - Azide and BSA Free

Western Blot: SRY Antibody (OTI3C8)Azide and BSA Free [NBP2-74352]

Western Blot: SRY Antibody (OTI3C8)Azide and BSA Free [NBP2-74352]

Western Blot: SRY Antibody (OTI3C8) - Azide and BSA Free [NBP2-74352] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRY.
Immunocytochemistry/ Immunofluorescence: SRY Antibody (OTI3C8) - Azide and BSA Free [NBP2-74352]

Immunocytochemistry/ Immunofluorescence: SRY Antibody (OTI3C8) - Azide and BSA Free [NBP2-74352]

Immunocytochemistry/Immunofluorescence: SRY Antibody (OTI3C8) - Azide and BSA Free [NBP2-74352] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY SRY.

Applications for SRY Antibody (OTI3C8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRY

SRY encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome.

Alternate Names

essential protein for sex determination in human males, sex determining region Y, sex-determining region on Y, sex-determining region Y protein, TDFsex determining region protein, TDY, Testis-determining factor

Gene Symbol

SRY

Additional SRY Products

Product Documents for SRY Antibody (OTI3C8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SRY Antibody (OTI3C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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