Key Product Details

Species Reactivity

Validated:

Human, Canine, Monkey, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C8

Concentration

0.8 mg/ml

View all SSB Antibodies »

Product Summary for SSB Antibody (OTI2C8)

Immunogen

Full-length protein expressed in 293T cell transfected with human SSB expression vector

Specificity

This antibody is specific for Homo sapiens Sjogren syndrome antigen B (autoantigen La) (SSB).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSB Antibody (OTI2C8)

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002]

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002]

Western Blot: SSB Antibody (OTI2C8) [NBP1-48002] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002]

Immunocytochemistry/ Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002]

Immunocytochemistry/Immunofluorescence: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.
Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002]

Immunohistochemistry-Paraffin: SSB Antibody (OTI2C8) [NBP1-48002] - Staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody.
View 12 more images

Applications for SSB Antibody (OTI2C8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:1000-2000
Application Notes
IP was tested using 2ug of antibody per 500ul at 0.5mg/ml overexpression lysate. Optimal dilutions for other samples will need to be determined by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.8 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SSB

La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaper

Alternate Names

La autoantigen, La ribonucleoprotein, lupus La protein, member 3, Sjoegren syndrome type B antigen, Sjogren syndrome antigen B (autoantigen La), SS-B, SS-B/La protein

Entrez Gene IDs

6741 (Human)

Gene Symbol

SSB

Additional SSB Products

Product Documents for SSB Antibody (OTI2C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SSB Antibody (OTI2C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.