SSEA-1 Antibody (BRA-4F1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45303
![Immunohistochemistry-Paraffin: SSEA-1 Antibody (BRA-4F1) [NBP2-45303]](https://resources.bio-techne.com/images/products/SSEA-1-Antibody-BRA-4F1-Immunohistochemistry-Paraffin-NBP2-45303-img0001.jpg "Immunohistochemistry-Paraffin: SSEA-1 Antibody (BRA-4F1) [NBP2-45303]")
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # BRA-4F1
Concentration
0.2 mg/ml
Product Summary for SSEA-1 Antibody (BRA-4F1)
Immunogen
Myelomonocytic leukemia cells
Localization
Cell surface and granular paranuclear
Marker
Reed-Sternberg Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11604)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for SSEA-1 Antibody (BRA-4F1)
Immunohistochemistry-Paraffin: SSEA-1 Antibody (BRA-4F1) [NBP2-45303]
Immunohistochemistry-Paraffin: SSEA-1 Antibody (BRA-4F1) [NBP2-45303] - Human Hodgkin's lymphoma stained with CD15 Monoclonal Antibody (BRA-4F1).Applications for SSEA-1 Antibody (BRA-4F1)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: SSEA-1
Long Name
Stage-specific Embryonic Antigen-1
Alternate Names
SSEA1
Gene Symbol
FUT4
UniProt
Additional SSEA-1 Products
Product Documents for SSEA-1 Antibody (BRA-4F1)
Product Specific Notices for SSEA-1 Antibody (BRA-4F1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...