SSEA-1 Antibody (MC-480) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80961
![Simple Western: SSEA-1 Antibody (MC-480)Azide and BSA Free [NBP2-80961]](https://resources.bio-techne.com/images/products/SSEA-1-Antibody-MC-480-Azide-and-BSA-Free-Simple-Western-NBP2-80961-img0004.jpg "Simple Western: SSEA-1 Antibody (MC-480)Azide and BSA Free [NBP2-80961]")
![Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]](https://resources.bio-techne.com/images/products/SSEA-1-Antibody-MC-480-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80961-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chimpanzee
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # MC-480
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for SSEA-1 Antibody (MC-480) - Azide and BSA Free
Immunogen
F9 tetracarcinoma stem cells (X-irradiated).
Reactivity Notes
This antibody recognizes SSEA-1 in mouse EC and ES cells but not human, but SSEA1 is expressed by some human differentiated cell types (ie: trophoblasts). Reactivity with Rat is reported by Kim et al. 2007 (PMID 17548530). Use in Chimpanzee reported in scientific literature (PMID:32045848).
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for SSEA-1 Antibody (MC-480) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]
Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red). Image from the standard format of this antibody.![Flow Cytometry: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]](https://resources.bio-techne.com/images/products/SSEA-1-Antibody-MC-480-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80961-img0003.jpg "Flow Cytometry: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]")
Flow Cytometry: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]
Flow Cytometry: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - An intracellular stain was performed on Jurkat cells with SSEA-1 Antibody (MC-480)NB100-1831 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]](https://resources.bio-techne.com/images/products/SSEA-1-Antibody-MC-480-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80961-img0001.jpg "Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]")
Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961]
Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (MC-480) - Azide and BSA Free [NBP2-80961] - SSEA1 antibody (0.1ug/ml) was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.Applications for SSEA-1 Antibody (MC-480) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
2.5 ug/million cells
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Frozen
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Simple Western
1:10
Western Blot
2 ug/ml
Application Notes
This SSEA1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Paraffin.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgM purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SSEA-1
Long Name
Stage-specific Embryonic Antigen-1
Alternate Names
SSEA1
Gene Symbol
FUT4
Additional SSEA-1 Products
Product Documents for SSEA-1 Antibody (MC-480) - Azide and BSA Free
Product Specific Notices for SSEA-1 Antibody (MC-480) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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