Key Product Details

Species Reactivity

Human, Mouse, Canine, Chicken, Primate, Rabbit

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # MC-813-70

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SSEA-4 Antibodies »

Product Specifications

Immunogen

Human embryonal carcinoma cell line 2102Ep

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for SSEA-4 Antibody (MC-813-70) [DyLight 350]

Application
Recommended Usage

Flow (Cell Surface)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SSEA-4

SSEAs were originally identified by monoclonal antibodies recognizing defined carbohydrate epitopes associated with lacto- and globo-series glycolipids. They are often used as markers for stem cell differentiation. CD15/Lewis X/SSEA-1 is expressed on murine embryonal carcinoma (EC) cells, embryonic stem (ES) cells, and primordial germ cells. SSEA-3 and SSEA-4 are synthesized during oogenesis and is present on oocyte, zygote, and early cleavage-stage embryo membranes. Mouse CD15/Lewis X/SSEA-1 expression decreases with differentiation as SSEA-3 and SSEA-4 expression increase. In contrast, human EC and ES cells express SSEA-3 and SSEA-4, and differentiation is accompanied by an upregulation of CD15/Lewis X/SSEA-1 and down-regulation of SSEA-3 and SSEA-4.

Long Name

Stage-specific Embryonic Antigen-4

Alternate Names

SSEA4

Additional SSEA-4 Products

Product Documents for SSEA-4 Antibody (MC-813-70) [DyLight 350]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SSEA-4 Antibody (MC-813-70) [DyLight 350]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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