SSX2 Antibody (1A4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006757-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SSX2 Antibodies »

Product Specifications

Immunogen

SSX2 (AAH02818, 1 a.a. - 223 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGNREAQEKEERRGTAHRWSSQNTHNIGRFSLSTSMGAVHGTPKTITHNRDPKGGNMPGPTDCVRENSW

Specificity

SSX2 - synovial sarcoma, X breakpoint 2 (1A4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SSX2 Antibody (1A4)

Western Blot: SSX2 Antibody (1A4) [H00006757-M01]

Western Blot: SSX2 Antibody (1A4) [H00006757-M01]

Western Blot: SSX2 Antibody (1A4) [H00006757-M01] - Analysis of SSX2 expression in transfected 293T cell line by SSX2 monoclonal antibody (M01), clone 1A4.Lane 1: SSX2 transfected lysate(25.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: SSX2 Antibody (1A4) [H00006757-M01]

Immunohistochemistry-Paraffin: SSX2 Antibody (1A4) [H00006757-M01]

Immunohistochemistry-Paraffin: SSX2 Antibody (1A4) [H00006757-M01] - Analysis of monoclonal antibody to SSX2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
ELISA: SSX2 Antibody (1A4) [H00006757-M01]

ELISA: SSX2 Antibody (1A4) [H00006757-M01]

ELISA: SSX2 Antibody (1A4) [H00006757-M01] - Detection limit for recombinant GST tagged SSX2 is approximately 0.03ng/ml as a capture antibody.

Applications for SSX2 Antibody (1A4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 5 using H00006757-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SSX2

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

Cancer/testis antigen 5.2, cancer/testis antigen family 5, member 2a, CT5.2a, HD21, HOM-MEL-40, MGC119055, MGC15364, MGC3884, protein SSX2, sarcoma, synovial, X-chromosome-related 2, SSX, SSX2A, SSX2B, synovial sarcoma, X breakpoint 2B, synovial sarcoma, X breakpoint 2CT5.2, Tumor antigen HOM-MEL-40

Entrez Gene IDs

6757 (Human)

Gene Symbol

SSX2

UniProt

AAH02818 (Human)

Additional SSX2 Products

Product Documents for SSX2 Antibody (1A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SSX2 Antibody (1A4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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