Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D10

Concentration

0.67 mg/ml

View all SSX2 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SSX2 expression vector

Specificity

This antibody is specific for Homo sapiens synovial sarcoma, X breakpoint 2 (SSX2), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSX2 Antibody (OTI4D10)

Western Blot: SSX2 Antibody (OTI4D10) [NBP1-48008]

Western Blot: SSX2 Antibody (OTI4D10) [NBP1-48008]

Western Blot: SSX2 Antibody (4D10) [NBP1-48008] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX2.
Immunocytochemistry/Immunofluorescence: SSX2 Antibody (4D10) [NBP1-48008] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX2.
Immunohistochemistry-Paraffin: SSX2 Antibody (OTI4D10) [NBP1-48008]

Immunohistochemistry-Paraffin: SSX2 Antibody (OTI4D10) [NBP1-48008]

Immunohistochemistry-Paraffin: SSX2 Antibody (OTI4D10) [NBP1-48008] - SSX2 Antibody (4D10) [NBP1-48008] - Staining of paraffin-embedded Kidney tissue using anti-SSX2mouse monoclonal antibody.
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Applications for SSX2 Antibody (OTI4D10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.67 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SSX2

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

Cancer/testis antigen 5.2, cancer/testis antigen family 5, member 2a, CT5.2a, HD21, HOM-MEL-40, MGC119055, MGC15364, MGC3884, protein SSX2, sarcoma, synovial, X-chromosome-related 2, SSX, SSX2A, SSX2B, synovial sarcoma, X breakpoint 2B, synovial sarcoma, X breakpoint 2CT5.2, Tumor antigen HOM-MEL-40

Entrez Gene IDs

6757 (Human)

Gene Symbol

SSX2

UniProt

Q16385 (Human)

Additional SSX2 Products

Product Documents for SSX2 Antibody (OTI4D10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SSX2 Antibody (OTI4D10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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