ST2/IL-33R Antibody (2154B) [Phycoerythrin]

Novus Biologicals, part of Bio-Techne | Catalog # FAB5232P

Novus Biologicals, part of Bio-Techne
Discontinued Product
FAB5232P has been discontinued. View all ST2/IL-33R products.

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2154B

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ST2/IL-33R Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST-2/ IL-33R
Lys19-Phe328
Accession # Q01638

Specificity

Detects human ST-2/IL-33R in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ST2/IL-33R Antibody (2154B) [Phycoerythrin]

ST2/IL-33R Antibody (2154B) [PE]

Detection of ST2/IL-33R in HEK293 cells by Flow Cytometry.

HEK293 cells transfected with Human ST-2 and eGFP (A) vs irrelevant (B) were stained with eGFP and Rabbit Anti-Human ST2/IL-33R PE-conjugated Monoclonal Antibody (Catalog # FAB5232P). View our protocol for Staining Membrane-associated Proteins.

Applications for ST2/IL-33R Antibody (2154B) [Phycoerythrin]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Sodium Phosphate and Sodium Azide. See Certificate of Analysis for details.

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ST2/IL-33R

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33's ability to signal through transmembrane ST2 (10, 13‑15).

References

  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
  4. Li, H. et al. (2000) Genomics 67:284.
  5. Schmitz, J. et al. (2005) Immunity 23:479.

  6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.

  10. Palmer, G. et al. (2008) Cytokine 42:358.
  11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  13. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Long Name

Interleukin 33 Receptor

Alternate Names

DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1

UniProt

Q01638

Additional ST2/IL-33R Products

Product Documents for ST2/IL-33R Antibody (2154B) [Phycoerythrin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ST2/IL-33R Antibody (2154B) [Phycoerythrin]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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