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ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00006483-M01

Novus Biologicals, part of Bio-Techne
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H00006483-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ST3GAL2 (NP_008858, 28 a.a. ~ 127 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HHSMATLPYLDSGALDGTHRVKLVPGYAGLQRLSKERLSGKSCACRRCMGDAGASDWFDSHFDGNISPVWTRENMDLPPDVQRWWMMLQPQFKSHNTNEV

Specificity

ST3GAL2 - ST3 beta-galactoside alpha-2,3-sialyltransferase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12)

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01]

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01]

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01] - ST3GAL2 monoclonal antibody (M01), clone 1E12 Analysis of ST3GAL2 expression in K-562.
Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01]

Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01]

Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01] - Analysis of monoclonal antibody to ST3GAL2 on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 1 ~ 10 ug/ml.
Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01]

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01]

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12) [H00006483-M01] - Analysis of ST3GAL2 expression in transfected 293T cell line by ST3GAL2 monoclonal antibody (M01), clone 1E12.Lane 1: ST3GAL2 transfected lysate (Predicted MW: 40.2 KDa).Lane 2: Non-transfected lysate.

Applications for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2

Alternate Names

Alpha 2,3-ST 2, Sialyltransferase 4B, SIAT4B, ST3GalA.2

Entrez Gene IDs

6483 (Human)

Gene Symbol

ST3GAL2

OMIM

607188 (Human)

UniProt

Additional ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Products

Product Documents for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (1E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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