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ST3GAL4 Antibody (1F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006484-M01

Novus Biologicals, part of Bio-Techne
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H00006484-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ST3GAL4 (NP_006269, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FYFPIPEKKEPCLQGEAESKASKLFGNYSRDQPIFLRLEDYFWVKTPSAYELPYGTKGSEDLLLRVLAITSSSIPKNIQSLRCRRCVVVGNGHRLRNSSL

Specificity

ST3GAL4 - ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (1F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ST3GAL4 Antibody (1F4)

Western Blot: ST3GAL4 Antibody (1F4) [H00006484-M01]

Western Blot: ST3GAL4 Antibody (1F4) [H00006484-M01]

Western Blot: ST3GAL4 Antibody (1F4) [H00006484-M01] - ST3GAL4 monoclonal antibody (M01), clone 1F4. Analysis of ST3GAL4 expression in MCF-7.
Sandwich ELISA: ST3GAL4 Antibody (1F4) [H00006484-M01] - Detection limit for recombinant GST tagged ST3GAL4 is 3 ng/ml as a capture antibody.

Applications for ST3GAL4 Antibody (1F4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ST3GAL4

Synthesis of alpha-2,3-linked sialic acid to Gal(beta-1,3)GalNAc is mediated by at least 3 distinct beta-galactoside alpha-2,3-sialyltransferases (EC 2.4.99.4), including ST3GAL4. In contrast, only a single gene encodes the beta-galactoside alpha-2,6-sialyltransferase (EC 2.4.99.1), ST6GAL1 (MIM 109675) (Chang et al., 1995 [PubMed 7655169]).[supplied by OMIM]

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 4

Alternate Names

CGS23, EC 2.4.99.9, Gal-NAc 6S, NANTA3, SAT-3, SIAT4, SIAT4C, ST-4, ST3Gal IV

Entrez Gene IDs

6484 (Human)

Gene Symbol

ST3GAL4

UniProt

Additional ST3GAL4 Products

Product Documents for ST3GAL4 Antibody (1F4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ST3GAL4 Antibody (1F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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