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ST8SIA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30530

Novus Biologicals, part of Bio-Techne
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NBP2-30530
NBP2-30530-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for ST8SIA5 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SGLYITHHYYDNVKPRPGFHAMPSEIFNFLHLHSRGILRVHTGTCS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ST8SIA5 Antibody

Immunocytochemistry/ Immunofluorescence: ST8SIA5 Antibody [NBP2-30530]

Immunocytochemistry/ Immunofluorescence: ST8SIA5 Antibody [NBP2-30530]

Immunocytochemistry/Immunofluorescence: ST8SIA5 Antibody [NBP2-30530] - Staining of human cell line RH-30 shows localization to plasma membrane & midbody. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530]

Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530]

Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530]

Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530]

Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Applications for ST8SIA5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ST8SIA5

ST8SIA5 - ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5

Alternate Names

alpha-2,8-sialyltransferase 8E, EC 2.4.99.-, MGC119670, Sialyltransferase 8E, sialyltransferase 8E (alpha-2, 8-polysialytransferase), Sialytransferase St8Sia V, SIAT8-E, SIAT8EMGC119671, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5, ST8SiaV

Gene Symbol

ST8SIA5

UniProt

Additional ST8SIA5 Products

Product Documents for ST8SIA5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ST8SIA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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