ST8SIA5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30530
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for ST8SIA5 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SGLYITHHYYDNVKPRPGFHAMPSEIFNFLHLHSRGILRVHTGTCS
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ST8SIA5 Antibody
Immunocytochemistry/ Immunofluorescence: ST8SIA5 Antibody [NBP2-30530]
Immunocytochemistry/Immunofluorescence: ST8SIA5 Antibody [NBP2-30530] - Staining of human cell line RH-30 shows localization to plasma membrane & midbody. Antibody staining is shown in green.Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530]
Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530] - Staining of human skin shows no positivity in squamous epithelial cells as expected.Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530]
Immunohistochemistry-Paraffin: ST8SIA5 Antibody [NBP2-30530] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.Applications for ST8SIA5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ST8SIA5
Alternate Names
alpha-2,8-sialyltransferase 8E, EC 2.4.99.-, MGC119670, Sialyltransferase 8E, sialyltransferase 8E (alpha-2, 8-polysialytransferase), Sialytransferase St8Sia V, SIAT8-E, SIAT8EMGC119671, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5, ST8SiaV
Gene Symbol
ST8SIA5
UniProt
Additional ST8SIA5 Products
Product Documents for ST8SIA5 Antibody
Product Specific Notices for ST8SIA5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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