STAG2 Antibody (3C6)

Novus Biologicals, part of Bio-Techne | Catalog # H00010735-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all STAG2 Antibodies »

Product Specifications

Immunogen

STAG2 (NP_006594.3, 1130 a.a. ~ 1231 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KRLRPEDSFMSVYPMQTEHHQTPLDYNRRGTSLMEDDEEPIVEDVMMSSEGRIEDLNEGMDFDTMDIDLPPSKNRRERTELKPDFFDPASIMDESVLGVSMF

Specificity

STAG2 - stromal antigen 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for STAG2 Antibody (3C6)

Western Blot: STAG2 Antibody (3C6) [H00010735-M01]

Western Blot: STAG2 Antibody (3C6) [H00010735-M01]

Western Blot: STAG2 Antibody (3C6) [H00010735-M01] - STAG2 monoclonal antibody (M01), clone 3C6 Analysis of STAG2 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: STAG2 Antibody (3C6) [H00010735-M01]

Immunocytochemistry/ Immunofluorescence: STAG2 Antibody (3C6) [H00010735-M01]

Immunocytochemistry/Immunofluorescence: STAG2 Antibody (3C6) [H00010735-M01] - Analysis of monoclonal antibody to STAG2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: STAG2 Antibody (3C6) [H00010735-M01]

Immunohistochemistry-Paraffin: STAG2 Antibody (3C6) [H00010735-M01]

Immunohistochemistry-Paraffin: STAG2 Antibody (3C6) [H00010735-M01] - Analysis of monoclonal antibody to STAG2 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
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Applications for STAG2 Antibody (3C6)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using H00010735-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STAG2

SA2 is a component of the cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which, sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The subunit interacts directly with RAD21 in cohesin complex. Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1L1 or SMC1L2) and SMC3, which are attached via their hinge domain, and RAD21 links them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG2 is mutually exclusive with STAG1 and STAG3.

Alternate Names

bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753

Entrez Gene IDs

10735 (Human)

Gene Symbol

STAG2

OMIM

604359 (Human)

UniProt

NP_006594 (Human)

Additional STAG2 Products

Product Documents for STAG2 Antibody (3C6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAG2 Antibody (3C6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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