STAG2 Antibody (3H4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15155
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3H4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for STAG2 Antibody (3H4)
Immunogen
A synthesized peptide derived from human STAG2. UniProt ID (Q8N3U4).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for STAG2 Antibody (3H4)
Western Blot: STAG2 Antibody (3H4) [NBP3-15155]
Western Blot: STAG2 Antibody (3H4) [NBP3-15155] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: STAG2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 142, 146 KDa. Observed band size: 142 KDa.Immunocytochemistry/ Immunofluorescence: STAG2 Antibody (3H4) [NBP3-15155]
Immunocytochemistry/Immunofluorescence: STAG2 Antibody (3H4) [NBP3-15155] - Immunofluorescence staining of MCF7 Cells with at STAG2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for STAG2 Antibody (3H4)
Application
Recommended Usage
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: STAG2
Alternate Names
bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753
Gene Symbol
STAG2
Additional STAG2 Products
Product Documents for STAG2 Antibody (3H4)
Product Specific Notices for STAG2 Antibody (3H4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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