STAG2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-301
![Western Blot: STAG2 Antibody [NB100-301]](https://resources.bio-techne.com/images/products/STAG2-Antibody-Western-Blot-NB100-301-img0007.jpg "Western Blot: STAG2 Antibody [NB100-301]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A 31 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 2 encoded in part by exon 31 and 32 (LocusLink ID 10735).
Specificity
NB100-301 is specific for human SA2 protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for STAG2 Antibody - BSA Free
Western Blot: STAG2 Antibody [NB100-301]
Western Blot: STAG2 Antibody [NB100-301] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody : Affinity purified rabbit anti-SA2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Immunocytochemistry/ Immunofluorescence: STAG2 Antibody [NB100-301]](https://resources.bio-techne.com/images/products/STAG2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-301-img0002.jpg "Immunocytochemistry/ Immunofluorescence: STAG2 Antibody [NB100-301]")
Immunocytochemistry/ Immunofluorescence: STAG2 Antibody [NB100-301]
Immunocytochemistry/Immunofluorescence: STAG2 Antibody [NB100-301] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.![Immunoprecipitation: STAG2 Antibody [NB100-301]](https://resources.bio-techne.com/images/products/STAG2-Antibody-Immunoprecipitation-NB100-301-img0010.jpg "Immunoprecipitation: STAG2 Antibody [NB100-301]")
Immunoprecipitation: STAG2 Antibody [NB100-301]
Immunoprecipitation: STAG2 Antibody [NB100-301] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA2 antibody used for IP at 6 ug per reaction. SA2 was also immunoprecipitated by rabbit anti-SA2 antibodies. For blotting immunoprecipitated SA2,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for STAG2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500-1:2000
Immunoprecipitation
2 - 10 ug/mg of lysate
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: STAG2
Alternate Names
bA517O1.1, cohesin subunit SA-2, DKFZp686P168, SA-2, SA2FLJ25871, SCC3 homolog 2, stromal antigen 2DKFZp781H1753
Gene Symbol
STAG2
Additional STAG2 Products
Product Documents for STAG2 Antibody - BSA Free
Product Specific Notices for STAG2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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