STAM2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2838

Novus Biologicals, part of Bio-Techne
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NB100-2838
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all STAM2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QSIDPTDSKPDSQD corresponding to internal region according to NP_005834.3.

Specificity

No cross-reaction expected with STAM1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for STAM2 Antibody

Western Blot: STAM2 Antibody [NB100-2838]

Western Blot: STAM2 Antibody [NB100-2838]

Western Blot: STAM2 Antibody [NB100-2838] - Staining of Human Spleen lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
STAM2 Antibody

Flow Cytometry: Goat Polyclonal STAM2 Antibody [NB100-2838] -

Flow Cytometry: Goat Polyclonal STAM2 Antibody [NB100-2838] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for STAM2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/mL
Application Notes
Approx 70kDa band observed in Human Spleen lysates and in preliminary testing of HeLa cell lysate (calculated MW of 58.2kDa according to NP_005834.3). This molecular weight is routinely observed by other sources.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAM2

The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation.

Alternate Names

DKFZp564C047, HBP, Hrs-binding protein, HSE1 homolog, signal transducing adapter molecule 2, signal transducing adaptor molecule (SH3 domain and ITAM motif) 2, STAM-2, STAM2A, STAM2B, STAM-like protein containing SH3 and ITAM domains 2

Entrez Gene IDs

10254 (Human); 56324 (Mouse)

Gene Symbol

STAM2

UniProt

NP_005834.3 (Human)

Additional STAM2 Products

Product Documents for STAM2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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