STAMP2/STEAP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68162

Novus Biologicals, part of Bio-Techne
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NB100-68162
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all STAMP2/STEAP4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CVDNTLTRIRQGWERN corresponding to C-Terminus according to NP_078912.2.

Reactivity Notes

Mouse reactivity reported in (PMID:29078383).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAMP2/STEAP4 Antibody

Western Blot: STAMP2/STEAP4 Antibody [NB100-68162]

Western Blot: STAMP2/STEAP4 Antibody [NB100-68162]

Western Blot: STAMP2/STEAP4 Antibody [NB100-68162] - HEK293 overexpressing Dudulin4 and probed with (mock transfection in first lane).
Western Blot: STAMP2/STEAP4 Antibody [NB100-68162]

Western Blot: STAMP2/STEAP4 Antibody [NB100-68162]

Western Blot: STAMP2/STEAP4 Antibody [NB100-68162] - (0.3ug/ml) Analysis of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoprecipitation: STAMP2/STEAP4 Antibody [NB100-68162]

Immunoprecipitation: STAMP2/STEAP4 Antibody [NB100-68162]

Immunoprecipitation: STAMP2/STEAP4 Antibody [NB100-68162] - Analysis of STEAP4 in MES13 cells using anti-STEAP4 antibody. Image from verified customer review.

Applications for STAMP2/STEAP4 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 50-55 kDa band observed in human adipose lysates (calculated MW of 52.0 kDa band according to NP_078912.2). In transfected HEK293 transiently expressing human Dudulin4 a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. IP reactivity per customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-68162 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAMP2/STEAP4

STEAP4 is a member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. It is similar to two other members of the STEAP family, STEAP2 and STEAP3. STEAP4 promotes the reduction of both iron (Fe3+ to Fe2+) and copper (Cu2+ to Cu1+). STEAP4 is highly expressed in placenta, lung, heart and prostate tissues. Furthermore, it is over expressed in prostate cancer cells compared to normal prostate cells. STEAP4 may have a role in cell proliferation and prostrate cancer progression since over expression of STEAP4 in prostate cells significantly increases cell growth and colony formation. At least two isoforms of this protein are known to exist.

Long Name

Six Transmembrane Protein of Prostate 2

Alternate Names

STEAP4, TIARP, TNFAIP9

Entrez Gene IDs

79689 (Human); 117167 (Mouse); 499991 (Rat)

Gene Symbol

STEAP4

UniProt

Q687X5 (Human)

Additional STAMP2/STEAP4 Products

Product Documents for STAMP2/STEAP4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAMP2/STEAP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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