StAR Antibody (STAR/3915R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13786
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/3915R) [NBP3-13786]](https://resources.bio-techne.com/images/products/StAR-Antibody-STAR-3915R-Immunohistochemistry-Paraffin-NBP3-13786-img0001.jpg "Immunohistochemistry-Paraffin: StAR Antibody (STAR/3915R) [NBP3-13786]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # STAR/3915R
Concentration
0.2 mg/ml
Product Summary for StAR Antibody (STAR/3915R)
Immunogen
Recombinant fragment (around aa 39-108) of human StAR protein (exact sequence is proprietary) (Uniprot: P49675)
Localization
Cytoplasm (Mitochondria)
Specificity
Steroidogenic Acute Regulatory Protein (STAR) controls the rate-limiting step of steroidegenesis by translocating cholesterol from the outer mitochondrial membrane to the inner membrane where it is later cleaved to pregnenolone. It is primarily present in steroid-producing cells, including Leydig cells in the testis, theca cells and luteal cells in the ovary and adrenal cells in the adrenal cortex. Due to low levels of pregnenolone, seminomas and Leydig cell tumors display no specific STAR staining. Therefore, STAR antibody may assist in differentiating sex cord stromal tumors (SCST), seminomas and embryonal carcinomas.
Marker
Leydig Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14189)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for StAR Antibody (STAR/3915R)
Immunohistochemistry-Paraffin: StAR Antibody (STAR/3915R) [NBP3-13786]
Immunohistochemistry-Paraffin: StAR Antibody (STAR/3915R) [NBP3-13786] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with StAR antibody (STAR/3915R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.Applications for StAR Antibody (STAR/3915R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: StAR
Alternate Names
cholesterol trafficker, mitochondrial steroid acute regulatory protein, StAR, StARD1, STARD1StAR-related lipid transfer (START) domain containing 1, START domain containing 1, START domain-containing protein 1, steroid acute regulatory protein, steroidogenic acute regulator, steroidogenic acute regulatory protein, steroidogenic acute regulatory protein, mitochondrial
Gene Symbol
STAR
UniProt
Additional StAR Products
Product Documents for StAR Antibody (STAR/3915R)
Product Specific Notices for StAR Antibody (STAR/3915R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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