STAT1 Antibody (OTI15H3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48013
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI15H3
Concentration
0.6 mg/ml
Product Specifications
Immunogen
Full-length protein expressed in 293T cell transfected with human STAT1 expression vector
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for STAT1 Antibody (OTI15H3)
Western Blot: STAT1 Antibody (OTI15H3) [NBP1-48013]
Western Blot: STAT1 Antibody (15H3) [NBP1-48013] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.
Immunocytochemistry/Immunofluorescence: STAT1 Antibody (15H3) [NBP1-48013] Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.
Immunohistochemistry-Paraffin: STAT1 Antibody (OTI15H3) [NBP1-48013]
Immunohistochemistry-Paraffin: STAT1 Antibody (15H3) [NBP1-48013] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STAT1 mouse monoclonal antibody.Applications for STAT1 Antibody (OTI15H3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.6 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: STAT1
Long Name
Signal Transducer and Activator of Transcription 1
Alternate Names
CANDF7, ISGF-3, STAT91
Entrez Gene IDs
6772 (Human)
Gene Symbol
STAT1
Additional STAT1 Products
Product Documents for STAT1 Antibody (OTI15H3)
Product Specific Notices for STAT1 Antibody (OTI15H3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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