STAT2 [p Tyr690] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-86975

Novus Biologicals, part of Bio-Techne
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NB110-86975
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all STAT2 Antibodies »

Product Summary for STAT2 [p Tyr690] Antibody

Immunogen

This affinity purified STAT2 [p Tyr690] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminus region near phospho Tyrosine 690 of human STAT2 protein. (Uniprot: P52630)

Reactivity Notes

Reactivity against homologues from other sources is not known

Modification

p Tyr690

Specificity

This affinity purified antibody is directed against human STAT2 pY690 protein. A BLAST analysis was used to suggest cross-reactivity with STAT2 pY690 protein from human, rat (73%) and mouse (76%), sources based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This affinity purified antibody is directed against human STAT2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for STAT2 [p Tyr690] Antibody

Western Blot: STAT2 [p Tyr690] Antibody [NB110-86975]

Western Blot: STAT2 [p Tyr690] Antibody [NB110-86975]

Western Blot: STAT2 [p Tyr690] Antibody [NB110-86975] - Analysis of Stat2pY690 protein (arrowhead) in Jurkat cells without (left lane) and with (right lane) 1000U/mL of IFN-a for 15 min at 37oC. Primary antibody was used at 1:1,000.
STAT2 [p Tyr690] Antibody

STAT2 [p Tyr690] Antibody

STAT2 [p Tyr690] Antibody showing nuclear positivity in epithelial cells of mouse urinary bladder tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-STAT2 pY690 antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
STAT2 [p Tyr690] Antibody

STAT2 [p Tyr690] Antibody

Western blot using Immunochemicals affinity purified anti-Stat2pY690 antibody shows detection of Stat2pY690 protein (arrowhead). Loaded Jurkat cells treated without (left lane) or with (right lane) 1000U/mL of IFN-a for 15 min at 37C. Primary antibody was used at 1:1,000. Personal Communication, A.Gamero, NCI, Bethesda, MD.

Applications for STAT2 [p Tyr690] Antibody

Application
Recommended Usage

ELISA

1:85000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500-1:1000
Application Notes
This affinity purified antibody has been tested for use in ELISA, western blotting, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 113 kDa in size corresponding to STAT2 pY690 protein by western blotting in the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: STAT2

STAT2 is a member of the STAT family of transcription factors. Unlike other STATs, STAT2 is unique as it can only be activated by interferons (IFNs). STAT2 is a critical component in mediating many IFN-stimulated biological activities including antiproliferation and antiviral responses. Upon IFN treatment, STAT1 and STAT2 become tyrosine phosphorylated, assemble as heterodimers that bind IRF9 to form the ISGF3 complex. This complex translocates to the nucleus, binds to promoters of IFNstimulated genes and mediates gene transcription. Consequently, mutations in STAT2 or loss of STAT2 expression leads to impairment in IFN signal transduction and gene activation. IFN-alpha is an approved drug for the treatment of several forms of cancer. Yet only a subset of patients who receive IFN-alpha therapy benefit from the treatment. Given that STAT2 is activated by IFNs, it is important to define if the reduced or lack of antitumor effects seen in cancer patients on IFN therapy is due to in defects in STAT2 function. Our goal is to identify regions/motifs within the structural domains of STAT2 that not only are essential for the tyrosine phosphorylation of STAT2, but also regulate the antitumor effects of IFN-alpha. Collectively, the results of our studies will emphasize the physiological role of STAT2 in cancer. From a clinical viewpoint, cancer patients who may benefit the most from receiving IFN-alpha therapy can be selected based on their STAT2 function.

Long Name

Signal Transducer and Activator of Transcription 2

Alternate Names

interferon alpha induced transcriptional activator, ISGF-3, MGC59816, p113, signal transducer and activator of transcription 2, signal transducer and activator of transcription 2, 113kD, signal transducer and activator of transcription 2, 113kDa, STAT113

Gene Symbol

STAT2

UniProt

P52630

Additional STAT2 Products

Product Documents for STAT2 [p Tyr690] Antibody

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Product Specific Notices for STAT2 [p Tyr690] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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