STAT5A [p Tyr694] Antibody (3F1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26387
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3F1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human STAT5A [p Tyr694] [UniProt P42229]
Modification
p Tyr 694
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for STAT5A [p Tyr694] Antibody (3F1)
![STAT5A [p Tyr694] Antibody (3F1)](https://resources.bio-techne.com/images/products/nbp3-26387_rabbit-stat5a-p-tyr-694-mab-3f1-262202414471572.jpg "Immunohistochemistry: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] -")
Immunohistochemistry: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] -
Immunohistochemistry: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] - Image of STAT5A [p Tyr694] Antibody (3F1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.![STAT5A [p Tyr694] Antibody (3F1)](https://resources.bio-techne.com/images/products/nbp3-26387_rabbit-stat5a-p-tyr-694-mab-3f1-282202411442631.jpg "Western Blot: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] -")
Western Blot: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] -
Western Blot: STAT5A [p Tyr694] Antibody (3F1) [NBP3-26387] - Positive Western Blot detected in HepG2 whole cell lysate (treated with EGF or not).All lanes: STAT5A [p Tyr694] Antibody at 1.025ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 90 KDa
Observed band size: 90 KDa
Applications for STAT5A [p Tyr694] Antibody (3F1)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: STAT5a
Long Name
Signal Transducer and Activator of Transcription 5
Alternate Names
MGF, signal transducer and activator of transcription 5A, STAT5
Gene Symbol
STAT5A
Additional STAT5a Products
Product Documents for STAT5A [p Tyr694] Antibody (3F1)
Product Specific Notices for STAT5A [p Tyr694] Antibody (3F1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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