STAT5A Antibody (6D4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37441

Novus Biologicals, part of Bio-Techne
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NBP2-37441
NBP2-37441-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6D4

Format

BSA Free

Concentration

1 mg/ml

View all STAT5a Antibodies »

Product Summary for STAT5A Antibody (6D4) - BSA Free

Immunogen

Purified recombinant fragment of human STAT5A (AA: 583-794) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

90.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT5A Antibody (6D4) - BSA Free

Western Blot: STAT5A Antibody (6D4)BSA Free [NBP2-37441]

Western Blot: STAT5A Antibody (6D4)BSA Free [NBP2-37441]

Western Blot: STAT5a Antibody (6D4) [NBP2-37441] - Western blot analysis using STAT5A mouse mAb against K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

Immunocytochemistry/ Immunofluorescence: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

Immunocytochemistry/Immunofluorescence: STAT5a Antibody (6D4) [NBP2-37441] - Immunofluorescence analysis of Hela cells using STAT5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

Immunohistochemistry: STAT5A Antibody (6D4) - BSA Free [NBP2-37441]

Immunohistochemistry: STAT5a Antibody (6D4) [NBP2-37441] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using STAT5A mouse mAb with DAB staining.
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Applications for STAT5A Antibody (6D4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT5a

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Gene Symbol

STAT5A

Additional STAT5a Products

Product Documents for STAT5A Antibody (6D4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAT5A Antibody (6D4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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