STAT5a/b Antibody (3J2A2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16533
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3J2A2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 600-786 of human STAT5a/b (P42229). KQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYMDQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLF
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for STAT5a/b Antibody (3J2A2)
![Western Blot: STAT5a/b Antibody (3J2A2) [NBP3-16533]](https://resources.bio-techne.com/images/products/STAT5a-b-Antibody-3J2A2-Western-Blot-NBP3-16533-img0001.jpg "Western Blot: STAT5a/b Antibody (3J2A2) [NBP3-16533]")
Western Blot: STAT5a/b Antibody (3J2A2) [NBP3-16533]
Western Blot: STAT5a/b Antibody (3J2A2) [NBP3-16533] - Western blot analysis of extracts of various cell lines, using STAT5a/b Rabbit mAb (NBP3-16533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Applications for STAT5a/b Antibody (3J2A2)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: STAT5a/b
Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.
Long Name
Signal Transducer and Activator of Transcription 5b
Alternate Names
MGF, STAT5, STAT5A signal transducer and activator of transcription 5A
Gene Symbol
STAT5A
Additional STAT5a/b Products
Product Documents for STAT5a/b Antibody (3J2A2)
Product Specific Notices for STAT5a/b Antibody (3J2A2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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