STAT6 Antibody (177C322.1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25241

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 177C322.1

Format

BSA Free

Concentration

1.0 mg/ml

View all STAT6 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 600-650 of human Signal Transducer and Activator of Transcription 6 was used as the immunogen for the STAT6 Antibody (177C322.1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for STAT6 Antibody (177C322.1) - BSA Free

Western Blot: STAT6 Antibody (177C322.1)BSA Free [NBP2-25241]

Western Blot: STAT6 Antibody (177C322.1)BSA Free [NBP2-25241]

Western Blot: STAT6 Antibody (177C322.1) [NBP2-25241] - Analysis of STAT6 in HeLa lysate in the A) absence and B) presence of immunizing peptide using STAT6 antibody at 2 ug/ml. anti mouse Ig HRP secondary antibody and ECL substrate were used for this test.
Immunocytochemistry/ Immunofluorescence: STAT6 Antibody (177C322.1) - BSA Free [NBP2-25241]

Immunocytochemistry/ Immunofluorescence: STAT6 Antibody (177C322.1) - BSA Free [NBP2-25241]

Immunocytochemistry/Immunofluorescence: STAT6 Antibody (177C322.1) [NBP2-25241] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-STAT6 (177C322.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
STAT6 Antibody (177C322.1) - BSA Free

STAT6 (177C322.1) in A431 Human Cell Line.

STAT6 (177C322.1) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti- STAT6 (177C322.1) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-25241AF488) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for STAT6 Antibody (177C322.1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunoprecipitation

1-2 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Janus family tyrosine kinases (Jak)/ STAT signal transduction pathway and mediates cytokine signaling by IL 4 and IL-13 (1). STAT6 (predicted molecular weight 94kDa) has been found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL-4. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is tyrosine phosphorylated (Tyr641) in response to cellular interaction with IL-4. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 and Th9 immune response (2). Loss of STAT6 impairs IL-4 mediated functions including Th2 helper T cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. Diseases associated with STAT6 include malignant hemangiopericytoma and nut allergies.

References

1. Waqas, S. F. H., Ampem, G., & Roszer, T. (2019). Analysis of IL-4/STAT6 Signaling in Macrophages. Methods Mol Biol, 1966, 211-224. doi:10.1007/978-1-4939-9195-2_17

2. Goenka, S., & Kaplan, M. H. (2011). Transcriptional regulation by STAT6. Immunol Res, 50(1), 87-96. doi:10.1007/s12026-011-8205-2

Long Name

Signal Transducer and Activator of Transcription 6

Alternate Names

D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT

Gene Symbol

STAT6

UniProt

NP_003144

Additional STAT6 Products

Product Documents for STAT6 Antibody (177C322.1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAT6 Antibody (177C322.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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