Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Staufen Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Staufen, RNA Binding Protein, Homolog 1 using the numbering given in entry NP_059347.2 (GeneID 6780).

Reactivity Notes

Panda (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Staufen Antibody - BSA Free

Western Blot: Staufen Antibody [NBP2-22308]

Western Blot: Staufen Antibody [NBP2-22308]

Western Blot: Staufen Antibody [NBP2-22308] - Whole cell lysate (50 ug) from 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. NBP2-22308 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Staufen Antibody [NBP2-22308]

Immunoprecipitation: Staufen Antibody [NBP2-22308]

Immunoprecipitation: Staufen Antibody [NBP2-22308] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22308 used for IP at 6 ug/mg lysate. Staufen1 was also immunoprecipitated by rabbit anti-Staufen1 antibody. For blotting immunoprecipitated Staufen1, NBP2-22308 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Staufen Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Staufen

Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR. [provided by RefSeq]

Alternate Names

double-stranded RNA-binding protein Staufen homolog 1, FLJ25010, RNA binding protein (Drosophila), RNA-binding protein), staufen, RNA binding protein, homolog 1 (Drosophila)

Gene Symbol

STAU1

UniProt

NP_059347.2 (Human)

Additional Staufen Products

Product Documents for Staufen Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Staufen Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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