STEP Antibody (23E5)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-202

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 23E5

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all STEP Antibodies »

Product Specifications

Immunogen

A synthetic peptide of rat STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)

Localization

Cytoplasmic

Specificity

NB300-202 is specific for STEP61, STEP46, STEP38, and STEP20.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for STEP Antibody (23E5)

Western Blot: STEP Antibody (23E5) [NB300-202]

Western Blot: STEP Antibody (23E5) [NB300-202]

Western Blot: STEP Antibody (23E5) [NB300-202] - Analysis of STEP protein in striatal rat protein homogenates
Immunohistochemistry-Paraffin: STEP Antibody (23E5) [NB300-202]

Immunohistochemistry-Paraffin: STEP Antibody (23E5) [NB300-202]

Immunohistochemistry-Paraffin: STEP Antibody (23E5) [NB300-202] - STEP was detected in immersion fixed paraffin-embedded sections of human brain basal ganglia using Mouse Anti-Human STEP (23E5) Monoclonal Antibody (Catalog # NB300-202) at 1:100 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in neurons.

Applications for STEP Antibody (23E5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunoprecipitation

1:50

Western Blot

1:1000 -1:2000
Application Notes
This STEP (23E5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. By Western Blot, this STEP antibody recognizes bands at ~64-66, 46, 37, and 33 kDa, in rat brain tissues. Neuronal cells have been used for ICC/IF and frozen brain sections have been used for IHC.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB300-202 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STEP

STEP (STriatal-Enriched protein tyrosine Phosphatase; PTPN5) is a brain-specific phosphatase enzyme which is regarded as an "LTD protein" because it mediates AMPA receptor internalization during metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD). STEP regulates MAPKs, Src kinases and NMDA receptors which are involved in synaptic plasticity as well as in neuronal cell survival mechanisms. STEP is highly expressed in striatum and is also found in hippocampus, cortex, amygdala, optic nerve, and spinal cord, but not expressed in cerebellum. PKA mediated phosphorylation at Ser-245 site deactivates STEP whereas MAPKs regulated phosphorylation at Thr-255/Ser-268 stabilizes STEP through preventing its proteasomal degradation. Out of four alternatively spliced variants (STEP61, STEP46, STEP38, STEP20), two major isoforms namely STEP61 and STEP46, contains a signature consensus tyrosine phosphatase sequence, [I/V]HCxAGxxR[S/T]G, necessary for its catalytic activity, and a kinase-interacting motif (KIM) essential for substrate binding. During extrasynaptic NMDAR stimulation, a truncated STEP product, STEP33, is generated by calpain-mediated cleavage between residues Ser224 and Leu225 in the KIM domain leading to disruption of STEP's ability to associate with and dephosphorylate its substrates. Subsequently, proteolytic cleavage of STEP after extrasynaptic NMDAR stimulation results in p38 activation, and initiates cell death signaling cascade. STEP participates in dopaminergic, glutamatergic, and nicotinic signaling, thereby playing a key role in psychostimulant addiction and Alzheimer's disease.

Long Name

Tyrosine-protein phosphatase non-receptor type 5

Alternate Names

PTPN5

Entrez Gene IDs

84867 (Human); 29644 (Rat)

Gene Symbol

PTPN5

Additional STEP Products

Product Documents for STEP Antibody (23E5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STEP Antibody (23E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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