STI1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20512

Novus Biologicals, part of Bio-Techne
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NBP2-20512
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all STI1 Antibodies »

Product Summary for STI1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human STI1. The exact sequence is proprietary.

Localization

Cytoplasm (By similarity); Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STI1 Antibody

Western Blot: STI1 Antibody [NBP2-20512]

Western Blot: STI1 Antibody [NBP2-20512]

Western Blot: STIP1 Antibody [NBP2-20512] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: STI1 Antibody [NBP2-20512]

Immunocytochemistry/ Immunofluorescence: STI1 Antibody [NBP2-20512]

Immunocytochemistry/Immunofluorescence: STIP1 Antibody [NBP2-20512] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STIP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: STI1 Antibody [NBP2-20512]

Immunohistochemistry-Paraffin: STI1 Antibody [NBP2-20512]

Immunohistochemistry-Paraffin: STIP1 Antibody [NBP2-20512] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:500 dilution.

Applications for STI1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STI1

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM]

Long Name

Stress-induced Phosphoprotein 1

Alternate Names

HOP, IEF-SSP-3521, NY-REN-11, STI1L, STIP1

Gene Symbol

STIP1

UniProt

P31948 (Human)

Additional STI1 Products

Product Documents for STI1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for STI1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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