STING/TMEM173 Antibody (2922D) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18817

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-18817
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2922D

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all STING/TMEM173 Antibodies »

Product Summary for STING/TMEM173 Antibody (2922D) - Azide and BSA Free

Immunogen

Partial recombinant protein made to amino acids 215-379 of human TMEM173/STING (UniProt Q86WV6).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STING/TMEM173 Antibody (2922D) - Azide and BSA Free

Immunohistochemistry: STING/TMEM173 Antibody (2922D) - Azide and BSA Free [NBP3-18817]

Immunohistochemistry: STING/TMEM173 Antibody (2922D) - Azide and BSA Free [NBP3-18817]

Immunohistochemistry: STING/TMEM173 Antibody (2292D) - Azide and BSA Free [NBP3-18817] - STING/TMEM173 was detected in immersion fixed paraffin-embedded sections of mouse lung tissue using Rabbit Anti-Human STING/TMEM173 Monoclonal Antibody at 0.5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pneumocytes and alveolar cells. Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for STING/TMEM173 Antibody (2922D) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

3 ug/ml

Immunohistochemistry

0.5 ug/ml

Western Blot

1 - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: STING/TMEM173

STING (stimulator of interferon genes) is encoded by the TMEM173 gene and is an adaptor molecule involved in the activation of innate immune responses to PAMPS (pathogen-associated molecular patterns) and DAMPS (damage-associated molecular patterns). STING specifically recognizes cytosolic DNA products derived from pathogens (e.g., cytomegalovirus, vaccinia virus, Listeria monocytogenes) or dead cells (1, 2). In the STING pathway, dsDNA derived from pathogens or damaged cells serves as a substrate for the enzyme cGAS (cyclic GMP-AMP synthase) which produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP (3, 4). Under steady-state conditions STING (theoretical molecular weight 42 kDa), a protein localizes to the ER membrane. Upon activation by dsDNA derived second messenger (cGAMP), STING translocates to the Golgi apparatus as a homodimer. Once STING has trafficked to the perinuclear region, it activates TANK binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3) and NF-kB leading to the production of cytokines (e.g., type I interferon) (2, 4). Mutations in the TMEM173 gene affecting STING expression are associated with the development of the auto-inflammatory disease SAVI (STING-associated vasculopathy with onset in infancy) (2). A novel SAVI dominant mutation in the TMEM173 human gene (V155M) leads to increased localization of STING to the Golgi and perinuclear region, indicative of an activated state (1). Hallmarks of SAVI, a rare inflammatory disease, include severe vasculitis in extremities and lung inflammation (7).

References

1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9

2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168

3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004

4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813

5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331

Long Name

Stimulator of Interferon Genes Protein/Transmembrane protein 173

Alternate Names

ERIS, MITA, MPYS, NET23, TMEM173, anti-STING, human STING, mouse STING, STING monoclonal

Gene Symbol

STING1

Additional STING/TMEM173 Products

Product Documents for STING/TMEM173 Antibody (2922D) - Azide and BSA Free

Product Datasheets

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Product Specific Notices for STING/TMEM173 Antibody (2922D) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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