STK32A Antibody (3E8-1D3)

Novus Biologicals, part of Bio-Techne | Catalog # H00202374-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E8-1D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all STK32A Antibodies »

Product Specifications

Immunogen

STK32A (AAH21666, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGANTSRKPPVFDENEDVNFDHFEILRAIGKGSFGKVCIVQKNDTKKMYAMKYMNKQKCVERNEVRNVFKELQIMQGLEHPFLVNLWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVHFKEETVKLFICELVMALDYLQNQRIIHRDMKPDNILLDEHDTWLSYKSH

Reactivity Notes

Human. Other species not tested.

Localization

Cytoplasm. Cell membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).

Specificity

STK32A - serine/threonine kinase 32A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for STK32A Antibody (3E8-1D3)

Western Blot: STK32A Antibody (3E8-1D3) [H00202374-M01]

Western Blot: STK32A Antibody (3E8-1D3) [H00202374-M01]

Western Blot: STK32A Antibody (3E8-1D3) [H00202374-M01] - Analysis of STK32A over-expressed 293 cell line, cotransfected with STK32A Validated Chimera RNAi ( Cat # H00202374-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK32A monoclonal antibody (M01) clone 3E8-1D3 (Cat # H00202374-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: STK32A Antibody (3E8-1D3) [H00202374-M01]

Western Blot: STK32A Antibody (3E8-1D3) [H00202374-M01]

Western Blot: STK32A Antibody (3E8-1D3) [H00202374-M01] - Analysis of STK32A expression in transfected 293T cell line by STK32A monoclonal antibody (M01), clone 3E8-1D3.Lane 1: STK32A transfected lysate(19.8 KDa).Lane 2: Non-transfected lysate.

Applications for STK32A Antibody (3E8-1D3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STK32A

Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty 2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C terminus of the protein. Significant sequence divergence occurs at the N terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.

Alternate Names

A930015B13Rik, EC 2.7.11, EC 2.7.11.1, MGC22688, serine/threonine kinase 32A, YANK1serine/threonine-protein kinase 32A

Entrez Gene IDs

202374 (Human)

Gene Symbol

STK32A

UniProt

AAH21666 (Human)

Additional STK32A Products

Product Documents for STK32A Antibody (3E8-1D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STK32A Antibody (3E8-1D3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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