STK39 Antibody (OTI4E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48021
![Flow Cytometry: STK39 Antibody (OTI4E3) [NBP1-48021]](https://resources.bio-techne.com/images/products/STK39-Antibody-4E3-Flow-Cytometry-NBP1-48021-img0001.jpg "Flow Cytometry: STK39 Antibody (OTI4E3) [NBP1-48021]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI4E3
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human STK39 (NP_037365) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens serine threonine kinase 39 (STE20/SPS1 homolog, yeast) (STK39).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for STK39 Antibody (OTI4E3)
Western Blot: STK39 Antibody (4E3) [NBP1-48021] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STK39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
![Immunocytochemistry/ Immunofluorescence: STK39 Antibody (OTI4E3) [NBP1-48021]](https://resources.bio-techne.com/images/products/STK39-Antibody-4E3-Immunocytochemistry-Immunofluorescence-NBP1-48021-img0003.jpg "Immunocytochemistry/ Immunofluorescence: STK39 Antibody (OTI4E3) [NBP1-48021]")
Immunocytochemistry/ Immunofluorescence: STK39 Antibody (OTI4E3) [NBP1-48021]
Immunocytochemistry/Immunofluorescence: STK39 Antibody (4E3) [NBP1-48021] - Immunofluorescent staining of COS7 cells using anti-STK39 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]](https://resources.bio-techne.com/images/products/STK39-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48021-img0007.jpg "Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]")
Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]
Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Human Ovary tissue using anti-STK39 mouse monoclonal antibody.![Western Blot: STK39 Antibody (OTI4E3) [NBP1-48021]](https://resources.bio-techne.com/images/products/STK39-Antibody-4E3-Western-Blot-NBP1-48021-img0002.jpg "Western Blot: STK39 Antibody (OTI4E3) [NBP1-48021]")
![Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]](https://resources.bio-techne.com/images/products/STK39-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48021-img0004.jpg "Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]")
![Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]](https://resources.bio-techne.com/images/products/STK39-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48021-img0005.jpg "Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]")
![Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]](https://resources.bio-techne.com/images/products/STK39-Antibody-4E3-Immunohistochemistry-Paraffin-NBP1-48021-img0006.jpg "Immunohistochemistry-Paraffin: STK39 Antibody (OTI4E3) [NBP1-48021]")
Applications for STK39 Antibody (OTI4E3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:500-1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: STK39
Alternate Names
DCHTsmall intestine SPAK-like kinase, DKFZp686K05124, EC 2.7.11, EC 2.7.11.1, PASK, proline-alanine-rich STE20-related kinase, serine threonine kinase 39, Serine/threonine-protein kinase 39, SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast), STE20/SPS1 homolog, STE20/SPS1-related proline-alanine-rich protein kinase, Ste20-like protein kinase, Ste-20-related kinase
Entrez Gene IDs
27347 (Human)
Gene Symbol
STK39
Additional STK39 Products
Product Documents for STK39 Antibody (OTI4E3)
Product Specific Notices for STK39 Antibody (OTI4E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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