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stonin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90658

Novus Biologicals, part of Bio-Techne
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NBP1-90658-25ul
NBP1-90658

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RTPSVTEAPPWRATNPFLNETLQDVQPSPINPFSAFFEEQERRSQNSSISSTTGKSQRDSLIVIYQDAISFDDSSKTQSHSDAVEKLKQLQIDDPDHFGSATLPDDDPVAWIELDAHPPGSARSQPRDGWPMMLRIPEK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for stonin-2 Antibody

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658]

Immunohistochemistry-Paraffin: stonin-2 Antibody [NBP1-90658] - Analysis in human cerebral cortex and liver tissues using NBP1-90658 antibody. Corresponding STON2 RNA-seq data are presented for the same tissues.
Western Blot: stonin-2 Antibody [NBP1-90658]

Western Blot: stonin-2 Antibody [NBP1-90658]

Western Blot: stonin-2 Antibody [NBP1-90658] - Analysis in human cell line K562.
Immunocytochemistry/ Immunofluorescence: stonin-2 Antibody [NBP1-90658]

Immunocytochemistry/ Immunofluorescence: stonin-2 Antibody [NBP1-90658]

Immunocytochemistry/Immunofluorescence: stonin-2 Antibody [NBP1-90658] - Staining of human cell line A-431 shows positivity in nucleoli & cytoplasm. Antibody staining is shown in green.

Applications for stonin-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:5

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: stonin-2

Alternate Names

STN2, STNB, STNB2, stoned B homolog 2, stonin 2, stonin-2

Gene Symbol

STON2

Additional stonin-2 Products

Product Documents for stonin-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for stonin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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