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STT3A Antibody (4D4)

Novus Biologicals, part of Bio-Techne | Catalog # H00003703-M02

Novus Biologicals, part of Bio-Techne
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H00003703-M02

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ITM1 (NP_689926, 603 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRG

Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Specificity

ITM1 - integral membrane protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for STT3A Antibody (4D4)

Western Blot: STT3A Antibody (4D4) [H00003703-M02]

Western Blot: STT3A Antibody (4D4) [H00003703-M02]

Western Blot: STT3A Antibody (4D4) [H00003703-M02] - Analysis of ITM1 expression in transfected 293T cell line by ITM1 monoclonal antibody (M02), clone 4D4.Lane 1: ITM1 transfected lysate(80.5 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: STT3A Antibody (4D4) [H00003703-M02]

Immunohistochemistry-Paraffin: STT3A Antibody (4D4) [H00003703-M02]

Immunohistochemistry-Paraffin: STT3A Antibody (4D4) [H00003703-M02] - Analysis of monoclonal antibody to ITM1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
ELISA: STT3A Antibody (4D4) [H00003703-M02]

ELISA: STT3A Antibody (4D4) [H00003703-M02]

ELISA: STT3A Antibody (4D4) [H00003703-M02] - Detection limit for recombinant GST tagged ITM1 is approximately 1ng/ml as a capture antibody.

Applications for STT3A Antibody (4D4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STT3A

Asparagine-linked glycosylation (ALG) is one of the most common protein modification reactions in eukaryotic cells, as many proteins that are translocated across or integrated into the rough endoplasmic reticulum (RER) carry N-linked oligosaccharides. STT3A is a subunit of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. SST3A seems to be involved in the complex substrate specificity.

Alternate Names

Integral membrane protein 1EC 2.4.1.119, integral transmembrane protein 1, ITM1dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, MGC9042, Oligosaccharyl transferase subunit STT3A, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S.cerevisiae), STT3-AFLJ27038, TMCB5, transmembrane conserved, Transmembrane protein TMC

Entrez Gene IDs

3703 (Human)

Gene Symbol

STT3A

OMIM

601134 (Human)

Additional STT3A Products

Product Documents for STT3A Antibody (4D4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for STT3A Antibody (4D4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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