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STX17 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93968

Novus Biologicals, part of Bio-Techne
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NBP1-93968
NBP1-93968-25ul

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHINAGRTVQQLRSNIREIEKLCLKVRKDDLVLLKRMIDPVKEEASAATAEFLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGAFHTTEAEASSQSLTQ

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28665306). Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STX17 Antibody

Western Blot: STX17 Antibody [NBP1-93968]

Western Blot: STX17 Antibody [NBP1-93968]

Western Blot: STX17 Antibody [NBP1-93968] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX17 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry: STX17 Antibody [NBP1-93968]

Immunohistochemistry: STX17 Antibody [NBP1-93968]

Immunohistochemistry: STX17 Antibody [NBP1-93968] - Immunohistochemical detection of STX17 in liver tissue. After fixation in neutral buffered formalin for 24 h, liver samples of wild type mice were paraffin-embedded and stained for STX17 using rabbit polyclonal anti-STX17 (Novus Biologicals, Littleton, CO, USA; NBP1-93968). Heat-mediated antigen retrieval was performed in citrate buffer (pH 6.0). An accumulation of STX17-positive dots is detectable after starvation. Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/6/3/17), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: STX17 Antibody [NBP1-93968]

Immunohistochemistry-Paraffin: STX17 Antibody [NBP1-93968]

Immunohistochemistry-Paraffin: STX17 Antibody [NBP1-93968] - Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells.

Applications for STX17 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in (PMID: 24554770)

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Immunoprecipitation

Reported in (PMID: 24554770)

Simple Western

1:30

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STX17

Alternate Names

MGC102796, MGC126613, MGC126615, syntaxin 17, syntaxin-17

Gene Symbol

STX17

Additional STX17 Products

Product Documents for STX17 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for STX17 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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