SUG1 Antibody (PSH0-22)

Western Blot: SUG1 Antibody (PSH0-22) [NBP3-32865]

Western Blot: SUG1 Antibody (PSH0-22) [NBP3-32865] - Western blot analysis of SUG1 on different lysates with Rabbit anti-SUG1 antibody (NBP3-32865) at 1/1,000 dilution.

Lane 1: A431 cell lysate (20 ug/Lane)
Lane 2: MCF-7 cell lysate (20 ug/Lane)
Lane 3: Hela cell lysate (20 ug/Lane)
Lane 4: HepG2 cell lysate (20 ug/Lane)
Lane 5: 293T cell lysate (20 ug/Lane)
Lane 6: NIH/3T3 cell lysate (20 ug/Lane)
Lane 7: PC-12 cell lysate (20 ug/Lane)
Lane 8: Mouse testis tissue lysate (40 ug/Lane)
Lane 9: Mouse heart tissue lysate (40 ug/Lane)
Lane 10: Rat testis tissue lysate (40 ug/Lane)
Lane 11: Rat brain tissue lysate (40 ug/Lane)

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32865) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: SUG1 Antibody (PSH0-22) [NBP3-32865]

Immunohistochemistry: SUG1 Antibody (PSH0-22) [NBP3-32865] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-SUG1 antibody (NBP3-32865) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32865) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.