Sulfatase-2/SULF2 Antibody (2B4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36727

Novus Biologicals, part of Bio-Techne
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NBP1-36727
NBP1-36727SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B4

Format

BSA Free

Concentration

1 mg/ml

View all Sulfatase-2/SULF2 Antibodies »

Product Specifications

Immunogen

Partial recombinant human Sulfatase 2 isoform b [Accession# NP_940998].

Localization

Endoplasmic reticulum

Specificity

Does not recognize human or mouse Sulfatase 1 or other family members.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Sulfatase-2/SULF2 Antibody (2B4) - BSA Free

Western Blot: Sulfatase-2/SULF2 Antibody (2B4)BSA Free [NBP1-36727]

Western Blot: Sulfatase-2/SULF2 Antibody (2B4)BSA Free [NBP1-36727]

Western Blot: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis showing Sulfatase 2 expression in MCF7 conditioned media (CM). In CM, the 75 kDa band is dominant (Tang R, Rosen SD. J Biol Chem. 2009 Aug 7;284(32):21505-14). Image courtesy of Mark Singer at UCSF.
Immunohistochemistry-Paraffin: Sulfatase-2/SULF2 Antibody (2B4) - BSA Free [NBP1-36727]

Immunohistochemistry-Paraffin: Sulfatase-2/SULF2 Antibody (2B4) - BSA Free [NBP1-36727]

Immunohistochemistry-Paraffin: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Immunohistochemical analysis of Sulfatase 2 in non-small-cell lung carcinoma. (left) lung squamous cell carcinoma stained with 2B4 antibody and (right) tumor-associated stromal cells stained with 2B4 antibody. Image courtesy of Mark Singer at UCSF.
Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) - BSA Free [NBP1-36727]

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) - BSA Free [NBP1-36727]

Flow Cytometry: Sulfatase-2/SULF2 Antibody (2B4) [NBP1-36727] - Analysis using the FITC conjugate of NBP1-36727. Staining of Human lung cancer line with Sulfatase2-FITC. Image from verified customer review.
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Applications for Sulfatase-2/SULF2 Antibody (2B4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

reported by customer review on FITC-conjugated antibody.

Immunohistochemistry

1:250-1:500

Immunohistochemistry-Paraffin

1:250-1:500

Immunoprecipitation

1:10-1:100

Western Blot

1:1000
Application Notes
For WB a band can be seen at 50 kDa. Other bands may be seen at 135 kDa (unprocessed protein) and 37 kDa (fragment of 50 kDa subunit). For ELISA, use this antibody at 1-10 ug/ml for capture and 1-5 ug/ml for detection. It has been reported that NBP1-36727 picks up a 130 kDa band that is not Sulfatase 2 in human plasma. Because of this we do not recommend NBP1-36727 for capture of the human protein. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sulfatase-2/SULF2

Sulfatase 2 (SULF2) is a member of sulfatase family and catalyzes the removal of 6-O-sulfate groups from the heparan sulfate (HS) disaccharide units of heparan sulfate proteoglycans (HSPGs). HSPGs are present on the cell surface as well as in the extracellular matrix and act either as sequestration sites for growth factor/cytokine ligands or as coreceptors in multiple signal transduction pathways. SULF2 mediated desulfation of HSPGs is proposed to decrease the affinity of HSPGs for a variety of signaling ligands, releasing them from sequestration sites and making them available for triggering signaling stimuli. SULF2 is localized in endoplasmic reticulum, Golgi apparatus and also on the cell surface, and is expressed at highest levels in tissues such as ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. In its post-translational modification, the conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. SULF2 enzyme regulates the activities of a number of signal transduction pathways which are up-regulated in many tissues during development and disease.

Alternate Names

SULF2, Sulfatase2

Entrez Gene IDs

55959 (Human); 72043 (Mouse)

Gene Symbol

SULF2

UniProt

NP_940998

Additional Sulfatase-2/SULF2 Products

Product Documents for Sulfatase-2/SULF2 Antibody (2B4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sulfatase-2/SULF2 Antibody (2B4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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