SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15317
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0L2N2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SUMO Activating Enzyme E1 (SAE1) (Q9UBE0). DSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)
![Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-0L2N2-Western-Blot-NBP3-15317-img0001.jpg "Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]")
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317] - Western blot analysis of extracts of various cell lines, using SUMO Activating Enzyme E1 (SAE1) Rabbit mAb (NBP3-15317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-0L2N2-Western-Blot-NBP3-15317-img0002.jpg "Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]")
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317]
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2) [NBP3-15317] - Western blot analysis of extracts of various cell lines, using SUMO Activating Enzyme E1 (SAE1) Rabbit mAb (NBP3-15317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.Applications for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SUMO Activating Enzyme E1 (SAE1/UBA2)
Long Name
SUMO1 Activating Enzyme E1
Alternate Names
AOS1, SAE1, SUA1, UBA2, UBLE1A
Gene Symbol
SAE1
Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products
Product Documents for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)
Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) Antibody (0L2N2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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