SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5)

Novus Biologicals, part of Bio-Techne | Catalog # H00010055-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 1G4-1G5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SUMO Activating Enzyme E1 (SAE1/UBA2) Antibodies »

Product Specifications

Immunogen

SAE1 (AAH00344, 1 a.a. ~ 346 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK

Reactivity Notes

Other species not tested.

Specificity

SAE1 - SUMO-1 activating enzyme subunit 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5)

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - SAE1 monoclonal antibody (M01), clone 1G4-1G5 Analysis of SAE1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01]

Immunocytochemistry/ Immunofluorescence: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01]

Immunocytochemistry/Immunofluorescence: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - Analysis of monoclonal antibody to SAE1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01]

Immunohistochemistry-Paraffin: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01]

Immunohistochemistry-Paraffin: SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5) [H00010055-M01] - Analysis of monoclonal antibody to SAE1 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.
View 1 more images

Applications for SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Long Name

SUMO1 Activating Enzyme E1

Alternate Names

AOS1, SAE1, SUA1, UBA2, UBLE1A

Entrez Gene IDs

10055 (Human)

Gene Symbol

SAE1

UniProt

AAH00344 (Human)

Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products

Product Documents for SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) Antibody (1G4-1G5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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