SUMO Activating Enzyme E1 (SAE1) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13272

Novus Biologicals, part of Bio-Techne
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NBP2-13272
NBP2-13272-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SUMO Activating Enzyme E1 (SAE1/UBA2) Antibodies »

Product Summary for SUMO Activating Enzyme E1 (SAE1) Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: NSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) Antibody

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272] - Analysis using Anti-SAE1 antibody NBP2-13272 (A) shows similar pattern to independent antibody NBP2-13273 (B).
Immunohistochemistry-Paraffin: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272]

Immunohistochemistry-Paraffin: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272]

Immunohistochemistry-Paraffin: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272] - Staining in human testis and pancreas tissues using anti-SAE1 antibody. Corresponding SAE1 RNA-seq data are presented for the same tissues.
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-13272] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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Applications for SUMO Activating Enzyme E1 (SAE1) Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunofluorescence, Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Long Name

SUMO1 Activating Enzyme E1

Alternate Names

AOS1, SAE1, SUA1, UBA2, UBLE1A

Gene Symbol

SAE1

Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products

Product Documents for SUMO Activating Enzyme E1 (SAE1) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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