SUMO-interacting Motif (SIM) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55180

Novus Biologicals, part of Bio-Techne
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NBP2-55180
NBP2-55180-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SUMO-interacting Motif (SIM) Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: YVIDLTRAEGENRPIATLDLTLEPVTPSQREPTSLQTCASLSGKAVMEGQVDRSSQPTARRLINSDPVDLDLVEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUMO-interacting Motif (SIM) Antibody

Immunocytochemistry/ Immunofluorescence: SUMO-interacting Motif (SIM) Antibody [NBP2-55180]

Immunocytochemistry/ Immunofluorescence: SUMO-interacting Motif (SIM) Antibody [NBP2-55180]

Immunocytochemistry/Immunofluorescence: SUMO-interacting Motif (SIM) Antibody [NBP2-55180] - Staining of human cell line CACO-2 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SUMO-interacting Motif (SIM) Antibody [NBP2-55180]

Immunohistochemistry-Paraffin: SUMO-interacting Motif (SIM) Antibody [NBP2-55180]

Immunohistochemistry-Paraffin: SUMO-interacting Motif (SIM) Antibody [NBP2-55180] - Staining of human bronchus shows strong cytoplasmic, nucleolar and membranous positivity in respiratory epithelial cells.

Applications for SUMO-interacting Motif (SIM) Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-P Retrieval method: HIER pH6.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUMO-interacting Motif (SIM)

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that are enzymatically attached to target proteins by a process termed SUMOylation. This post-translational modification regulates many cellular processes including DNA transcription and repair, cell cycle progression, protein intracellular trafficking, and nuclear receptor activities. SUMO binding and/or interaction with proteins is mediated by short amino acid consensus sequences termed SUMO-interacting motifs (SIMs). To date, all SIMs appear to contain a hydrophobic core sequence that is either preceded or succeeded by an acidic region composed of either glutamate or aspartate residues or phosphorylated serine or threonine residues. The hydrophobic core of SIMs has been shown to interact with the alpha-helix and beta2-strand surfaces on SUMO proteins while the negatively charged residues surrounding the hydrophobic core appear to influence binding affinities and dictate binding preferences for the various SUMO isoforms. SIMs have been identified in numerous types of proteins including SUMO ligases (E3), transcription factors, and transcriptional repressors.

Alternate Names

SUMOinteracting Motif

Gene Symbol

SIMC1

Additional SUMO-interacting Motif (SIM) Products

Product Documents for SUMO-interacting Motif (SIM) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUMO-interacting Motif (SIM) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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