SUMO1 Antibody (SPM571)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32902
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SPM571) [NBP2-32902]](https://resources.bio-techne.com/images/products/SUMO1-Antibody-SPM571-Immunohistochemistry-Paraffin-NBP2-32902-img0005.jpg "Immunohistochemistry-Paraffin: SUMO1 Antibody (SPM571) [NBP2-32902]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM571
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human SUMO1 protein (Uniprot: P63165)
Reactivity Notes
Shows broad species reactivity.
Localization
Predominantly nuclear with some cytoplasmic
Specificity
This monoclonal antibody is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34805)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for SUMO1 Antibody (SPM571)
Immunohistochemistry-Paraffin: SUMO1 Antibody (SPM571) [NBP2-32902]
Immunohistochemistry-Paraffin: SUMO1 Antibody (SPM571) [NBP2-32902] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with SUMO1 Antibody (SPM571)Applications for SUMO1 Antibody (SPM571)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: SUMO1
Long Name
Small Ubiquitin-like Modifier 1
Alternate Names
PIC1, Sentrin, SMT3C, SMT3H3, UBL1
Gene Symbol
SUMO1
UniProt
Additional SUMO1 Products
Product Documents for SUMO1 Antibody (SPM571)
Product Specific Notices for SUMO1 Antibody (SPM571)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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