SUMO1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76737

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (93%), Porcine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SUMO1 Antibodies »

Product Summary for SUMO1 Antibody - BSA Free

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human sumo. The immunogen is located within the first 50 amino acids of Sumo. Amino Acid Squence: EAKPSTEDLGDKKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUMO1 Antibody - BSA Free

Western Blot: SUMO1 AntibodyBSA Free [NBP1-76737]

Western Blot: SUMO1 AntibodyBSA Free [NBP1-76737]

Western Blot: SUMO1 Antibody [NBP1-76737] - HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody - BSA Free [NBP1-76737]

Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody - BSA Free [NBP1-76737]

Immunocytochemistry/Immunofluorescence: SUMO1 Antibody [NBP1-76737] - Sumo in HL60 cells with Sumo antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody - BSA Free [NBP1-76737]

Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody - BSA Free [NBP1-76737]

Immunocytochemistry/Immunofluorescence: SUMO1 Antibody [NBP1-76737] - HL60 cells with Sumo 1 antibody at 5 ug/ml.

Applications for SUMO1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUMO1

The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.

Long Name

Small Ubiquitin-like Modifier 1

Alternate Names

PIC1, Sentrin, SMT3C, SMT3H3, UBL1

Gene Symbol

SUMO1

UniProt

AAH66306 (Human)

Additional SUMO1 Products

Product Documents for SUMO1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUMO1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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