SUMO2/3 Antibody (10F1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22546

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 10F1

Format

BSA Free

Concentration

1 mg/ml

View all SUMO2/3 Antibodies »

Product Summary for SUMO2/3 Antibody (10F1) - BSA Free

Immunogen

Recombinant human SUMO2/3 (1-93aa) purified from E.coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for SUMO2/3 Antibody (10F1) - BSA Free

Western Blot: SUMO2/3 Antibody (10F1)BSA Free [NBP2-22546]

Western Blot: SUMO2/3 Antibody (10F1)BSA Free [NBP2-22546]

Western Blot: SUMO2/3 Antibody (10F1) [NBP2-22546] - Cell lysates of HeLa (35ug) and recombinant proteins (10ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SUMO2/3 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: SUMO2/3 Antibody (10F1) - BSA Free [NBP2-22546]

Immunocytochemistry/ Immunofluorescence: SUMO2/3 Antibody (10F1) - BSA Free [NBP2-22546]

Immunocytochemistry/Immunofluorescence: SUMO2/3 Antibody (10F1) [NBP2-22546] - Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human SUMO2/3 antibody (1:500) with Texas Red (Red).
Immunohistochemistry-Paraffin: SUMO2/3 Antibody (10F1) - BSA Free [NBP2-22546]

Immunohistochemistry-Paraffin: SUMO2/3 Antibody (10F1) - BSA Free [NBP2-22546]

Immunohistochemistry-Paraffin: SUMO2/3 Antibody (10F1) [NBP2-22546] - Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human SUMO2/3 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Applications for SUMO2/3 Antibody (10F1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:50

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUMO2/3

SUMO (small ubiquitin-related modifier) is the best-characterized member of a growing family of ubiquitin-related proteins. 8-11 kDa that covalently modify various intracellular proteins. It resembles ubiquitin in its structure, its ability to be ligated to other proteins. SUMO regulates cellular function of a variety of target proteins. SUMO proteins are expressed as their precursor forms. Cleavage of the residues after the 'GG' region of these precursors by SUMO-specific proteases in maturation is a prerequisite for subsequent sumoylation. Notably, SUMO2 and SUMO3 precursors have 96% sequence identity, and recent studies have shown protein substrates conjugated with SUMO2 or SUMO3 have similar, if not identical, biological consequences.

Long Name

Small Ubiquitin-like Modifier 2, & 3

Alternate Names

HSMT3, small ubiquitin-like modifier 2, SMT3A, SMT3B, SMT3H2, SUMO2, SUMO3

Gene Symbol

SUMO2

UniProt

NP_008868 (Human)

Additional SUMO2/3 Products

Product Documents for SUMO2/3 Antibody (10F1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SUMO2/3 Antibody (10F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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