SUMO4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97570
![Western Blot: SUMO4 Antibody [NBP2-97570]](https://resources.bio-techne.com/images/products/SUMO4-Antibody-Western-Blot-NBP2-97570-img0002.jpg "Western Blot: SUMO4 Antibody [NBP2-97570]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human SUMO4 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SUMO4 Antibody
Western Blot: SUMO4 Antibody [NBP2-97570]
Western Blot: SUMO4 Antibody [NBP2-97570] - Anti-SUMO4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 19 kDa![Immunocytochemistry/ Immunofluorescence: SUMO4 Antibody [NBP2-97570]](https://resources.bio-techne.com/images/products/SUMO4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97570-img0001.jpg "Immunocytochemistry/ Immunofluorescence: SUMO4 Antibody [NBP2-97570]")
Immunocytochemistry/ Immunofluorescence: SUMO4 Antibody [NBP2-97570]
Immunocytochemistry/Immunofluorescence: SUMO4 Antibody [NBP2-97570] - Immunofluorescence staining of SUMO4 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SUMO4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.Applications for SUMO4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SUMO4
Long Name
Small Ubiquitin-like Modifier 4
Alternate Names
dJ281H8.4, IDDM5, small ubiquitin-like modifier 4 protein, Small ubiquitin-like protein 4, small ubiquitin-related modifier 4, SMT3 suppressor of mif two 3 homolog 2, SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae), SMT3 suppressor of mif two 3 homolog 4 (yeast), SMT3H4, SUMO-4
Gene Symbol
SUMO4
Additional SUMO4 Products
Product Specific Notices for SUMO4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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