SUN1 Antibody (HL1947) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25702
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Chimpanzee (98%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1947
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SUN1 Antibody (HL1947) - Azide and BSA Free
Immunogen
Recombinant fragment of human SUN1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SUN1 Antibody (HL1947) - Azide and BSA Free
Knockdown Validated: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] -
Knockdown Validated: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] -
Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nuclear envelope by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .
Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] -
Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for SUN1 Antibody (HL1947) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SUN1
Alternate Names
KIAA0810UNC84AFLJ12407, MGC176649, Protein unc-84 homolog A, Sad1 and UNC84 domain containing 1, Sad1 unc-84 domain protein 1, Sad1/unc-84 protein-like 1, SUN domain-containing protein 1, unc-84 homolog A, unc-84 homolog A (C. elegans)
Gene Symbol
SUN1
Additional SUN1 Products
Product Documents for SUN1 Antibody (HL1947) - Azide and BSA Free
Product Specific Notices for SUN1 Antibody (HL1947) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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