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SUPT16H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38607

Novus Biologicals, part of Bio-Techne
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NBP2-38607
NBP2-38607-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ASITSEVFNKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFKSY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUPT16H Antibody

Immunocytochemistry/ Immunofluorescence: SUPT16H Antibody [NBP2-38607]

Immunocytochemistry/ Immunofluorescence: SUPT16H Antibody [NBP2-38607]

Immunocytochemistry/Immunofluorescence: SUPT16H Antibody [NBP2-38607] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607]

Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607]

Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607] - Staining in human testis and skeletal muscle tissues using anti-SUPT16H antibody. Corresponding SUPT16H RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607]

Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607]

Immunohistochemistry-Paraffin: SUPT16H Antibody [NBP2-38607] - Staining of human skeletal muscle shows low expression as expected.

Applications for SUPT16H Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUPT16H

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. (provided by RefSeq)

Alternate Names

CDC68, Chromatin-specific transcription elongation factor 140 kDa subunit, facilitates chromatin remodeling 140 kDa subunit, Facilitates chromatin transcription complex subunit SPT16, FACT 140 kDa subunit, FACT complex subunit SPT16, FACT140, FACTp140, FACTP140FACT, FLJ10857, FLJ14010, FLJ34357, hSPT16, SPT16/CDC68, suppressor of Ty (S.cerevisiae) 16 homolog, suppressor of Ty 16 homolog (S. cerevisiae)

Gene Symbol

SUPT16H

UniProt

Additional SUPT16H Products

Product Documents for SUPT16H Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SUPT16H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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