Survivin Antibody (S04-1B1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20088
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S04-1B1
Concentration
0.3 mg/ml
Product Specifications
Immunogen
Recombinant protein of human Survivin (Uniprot # O15392)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Survivin Antibody (S04-1B1)
![Western Blot: Survivin Antibody (S04-1B1) [NBP3-20088]](https://resources.bio-techne.com/images/products/Survivin-Antibody-S04-1B1-Western-Blot-NBP3-20088-img0002.jpg "Western Blot: Survivin Antibody (S04-1B1) [NBP3-20088]")
Western Blot: Survivin Antibody (S04-1B1) [NBP3-20088]
Western Blot: Survivin Antibody (S04-1B1) [NBP3-20088] - Western blot detection of Survivin in Jurkat cell lysates using NBP3-20088 (1:500 diluted). Predicted band size: 16kDa. Observed band size: 16kDa.
Immunocytochemistry/Immunofluorescence: Survivin Antibody (S04-1B1) [NBP3-20088] -
Immunocytochemistry/Immunofluorescence: Survivin Antibody (S04-1B1) [NBP3-20088] - Immunocytochemistry of Survivin (green) in MCF-7 using NBP3-20088 at dilution 1/20, and DAPI (blue)Applications for Survivin Antibody (S04-1B1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1/1000
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Survivin
Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).
Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).
/>References
/> 1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993
2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829
3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431
Alternate Names
API4, BIRC5
Gene Symbol
BIRC5
Additional Survivin Products
Product Documents for Survivin Antibody (S04-1B1)
Product Specific Notices for Survivin Antibody (S04-1B1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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