SUV420h1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97312
![Immunocytochemistry/ Immunofluorescence: SUV420h1 Antibody - BSA Free [NBP1-97312]](https://resources.bio-techne.com/images/products/SUV420h1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97312-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SUV420h1 Antibody - BSA Free [NBP1-97312]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot (Negative)
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.14 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human SUV420h1 protein (between residues 100-150) [UniProt Q4FZB7]
Localization
Nucleus. Chromosome. Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SUV420h1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: SUV420h1 Antibody - BSA Free [NBP1-97312]
Immunocytochemistry/Immunofluorescence: SUV420h1 Antibody [NBP1-97312] - SUV420h1 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).![Immunohistochemistry: SUV420h1 Antibody - BSA Free [NBP1-97312]](https://resources.bio-techne.com/images/products/SUV420h1-Antibody-Immunohistochemistry-NBP1-97312-img0004.jpg "Immunohistochemistry: SUV420h1 Antibody - BSA Free [NBP1-97312]")
Immunohistochemistry: SUV420h1 Antibody - BSA Free [NBP1-97312]
Immunohistochemistry: SUV420h1 Antibody [NBP1-97312] - IHC analysis of SUV420h1 in mouse epidermis using DAB with hematoxylin counterstain.Applications for SUV420h1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:300
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Application Notes
This SUV420h1 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in A431 cells. In IHC-P, staining was observed in the nucleus of mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.14 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SUV420h1
Long Name
Histone-lysine N-methyltransferase KMT5B
Alternate Names
CGI-85, KMT5B, Suv4-20h1
Entrez Gene IDs
51111 (Human)
Gene Symbol
KMT5B
UniProt
Additional SUV420h1 Products
Product Documents for SUV420h1 Antibody - BSA Free
Product Specific Notices for SUV420h1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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